|Other Names||Negative elongation factor B, NELF-B, Cofactor of BRCA1, NELFB, COBRA1, KIAA1182|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.|
|Tissue Location||Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
NELFB is a subunit of negative elongation factor (NELF),which also includes NELFA (WHSC2; MIM 606026), either NELFC orNELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF actswith DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4(SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to causetranscriptional pausing of RNA polymerase II (see MIM 180660)(Narita et al., 2003 [PubMed 12612062]).
Sun, J., et al. J. Cell. Biochem. 103(6):1798-1807(2008)Sun, J., et al. J. Steroid Biochem. Mol. Biol. 107 (3-5), 131-139 (2007) :Wu, C., et al. Proteomics 7(11):1775-1785(2007)Narita, T., et al. Mol. Cell 26(3):349-365(2007)Aiyar, S.E., et al. Int. J. Biol. Sci. 3(7):486-492(2007)
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