|Other Names||Cleavage and polyadenylation specificity factor subunit 3, 3127-, Cleavage and polyadenylation specificity factor 73 kDa subunit, CPSF 73 kDa subunit, mRNA 3'-end-processing endonuclease CPSF-73, CPSF3, CPSF73|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.|
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Provided below are standard protocols that you may find useful for product applications.
Most eukaryotic mRNA precursors (pre-mRNAs) undergoextensive maturational processing, including cleavage andpolyadenylation at the 3-prime end. CPSF3 encodes the 73-kD subunitof the cleavage and polyadenylation specificity factor (CPSF) andis the pre-mRNA 3-prime-end-processing endonuclease (Mandel et al.,2006 [PubMed 17128255]).
Rozenblatt-Rosen, O., et al. Proc. Natl. Acad. Sci. U.S.A. 106(3):755-760(2009)Zhu, Z.H., et al. Oncogene 28(1):41-51(2009)Yang, X.C., et al. Mol. Cell. Biol. 29(1):31-42(2009)Kolev, N.G., et al. EMBO Rep. 9(10):1013-1018(2008)de la Vega, L., et al. J. Mol. Biol. 372(2):317-330(2007)
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