|Other Names||FACT complex subunit SSRP1, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, FACT 80 kDa subunit, FACTp80, Facilitates chromatin transcription complex subunit SSRP1, Recombination signal sequence recognition protein 1, Structure-specific recognition protein 1, hSSRP1, T160, SSRP1, FACT80|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.|
|Cellular Location||Nucleus. Nucleus, nucleolus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci|
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The protein encoded by this gene is a subunit of aheterodimer that, along with SUPT16H, forms chromatintranscriptional elongation factor FACT. FACT interacts specificallywith histones H2A/H2B to effect nucleosome disassembly andtranscription elongation. FACT and cisplatin-damaged DNA may becrucial to the anticancer mechanism of cisplatin. This encodedprotein contains a high mobility group box which most likelyconstitutes the structure recognition element forcisplatin-modified DNA. This protein also functions as aco-activator of the transcriptional activator p63. An alternativelyspliced transcript variant of this gene has been described, but itsfull-length nature is not known.
Zeng, S.X., et al. Mol. Cell. Biol. 30(4):935-947(2010)Hautbergue, G.M., et al. Curr. Biol. 19(22):1918-1924(2009)Hu, J., et al. J. Virol. 83(21):11051-11063(2009)Kumari, A., et al. J. Cell. Biochem. 108(2):508-518(2009)Okada, M., et al. Mol. Biol. Cell 20(18):3986-3995(2009)
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