|Other Names||Protein-tyrosine kinase 6, SRC-related intestinal kinase, Ptk6, Sik|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage- independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.|
|Cellular Location||Cytoplasm. Nucleus. Membrane. Cell projection, ruffle. Note=Also found to be membrane- associated. Colocalizes with KHDRBS1, within the nucleus|
|Tissue Location||Expressed only in epithelial tissues, including the skin and lining of the alimentary canal. Restricted to the cell layers immediately above the proliferative cell zone in these epithelia.|
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Provided below are standard protocols that you may find useful for product applications.
Phosphorylates KHDRBS2, KHDRBS3 and STAP2/BKS (By similarity). Phosphorylates KHDRBS1. May function as an intracellular signal transducer in epithelial tissues.
Zheng, Y., et al. Mol. Cell. Biol. 30(17):4280-4292(2010)Palka-Hamblin, H.L., et al. J. Cell. Sci. 123 (PT 2), 236-245 (2010) :Xiang, B., et al. Proc. Natl. Acad. Sci. U.S.A. 105(34):12463-12468(2008)Whitehead, R.H., et al. J. Gastroenterol. Hepatol. 23 (7 PT 1), 1119-1124 (2008) :Haegebarth, A., et al. Mol. Cell. Biol. 26(13):4949-4957(2006)
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