|Other Names||E3 ubiquitin-protein ligase AMFR, 632-, Autocrine motility factor receptor, AMF receptor, RING finger protein 45, gp78, AMFR, RNF45|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
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Autocrine motility factor is a tumor motility-stimulatingprotein secreted by tumor cells. The protein encoded by this geneis a glycosylated transmembrane protein and a receptor forautocrine motility factor. The receptor, which shows some sequencesimilarity to tumor protein p53, is localized to the leading andtrailing edges of carcinoma cells.
Joshi, B., et al. J. Biol. Chem. 285(12):8830-8839(2010)Bernardi, K.M., et al. Mol. Biol. Cell 21(1):140-151(2010)Ballar, P., et al. Int. J. Biochem. Cell Biol. 42(1):167-173(2010)Shmueli, A., et al. Biochem. Biophys. Res. Commun. 390(3):758-762(2009)Ying, Z., et al. Hum. Mol. Genet. 18(22):4268-4281(2009)
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