TLR8 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9NR97 |
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Clone Names | 2103134 |
Gene ID | 51311 |
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Other Names | Toll-like receptor 8, CD288, TLR8 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP1508a was selected from the N-term region of human Human TLR8 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | TLR8 (HGNC:15632) |
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Function | Endosomal receptor that plays a key role in innate and adaptive immunity (PubMed:25297876, PubMed:32433612). Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2 (PubMed:31778653). Recognizes GU-rich single- stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV- 1 viruses (PubMed:33718825). Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction (PubMed:23520111, PubMed:25599397, PubMed:26929371, PubMed:33718825). In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF- kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively (PubMed:16737960, PubMed:17932028, PubMed:29155428). |
Cellular Location | Endosome membrane; Single-pass type I membrane protein. Note=Endosomal localization confers distinctive proteolytic processing |
Tissue Location | Expressed in myeloid dendritic cells, monocytes, and monocyte-derived dendritic cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
TLR8 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR8 is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X.
References
Heil, F., et al., Science 303(5663):1526-1529 (2004).Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).Miettinen, M., et al., Genes Immun. 2(6):349-355 (2001).Chuang, T.H., et al., Eur. Cytokine Netw. 11(3):372-378 (2000).Du, X., et al., Eur. Cytokine Netw. 11(3):362-371 (2000).
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