RAD1 Antibody (Center) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | O60671 |
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Clone Names | 100507263 |
Gene ID | 5810 |
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Other Names | Cell cycle checkpoint protein RAD1, hRAD1, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, RAD1, REC1 |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | RAD1 |
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Synonyms | REC1 |
Function | Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed:10846170, PubMed:10884395). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed:12578958). Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed:15871698). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed:15314187, PubMed:15556996, PubMed:15871698). The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed:21659603). |
Cellular Location | Nucleus. |
Tissue Location | Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a component of a heterotrimeric cellcycle checkpoint complex, known as the 9-1-1 complex, that isactivated to stop cell cycle progression in response to DNA damageor incomplete DNA replication. The 9-1-1 complex is recruited byRAD17 to affected sites where it may attract specialized DNApolymerases and other DNA repair effectors. Alternatively splicedtranscript variants of this gene have been described. [provided byRefSeq].
References
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)Takeishi, Y., et al. Genes Cells 15(7):761-771(2010)Bai, H., et al. DNA Repair (Amst.) 9(5):478-487(2010)Park, M.J., et al. DNA Repair (Amst.) 8(10):1190-1200(2009)Xu, M., et al. J. Biol. Chem. 284(31):20457-20461(2009)
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