|Other Names||Cell cycle checkpoint protein RAD1, hRAD1, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, RAD1, REC1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.|
|Tissue Location||Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a component of a heterotrimeric cellcycle checkpoint complex, known as the 9-1-1 complex, that isactivated to stop cell cycle progression in response to DNA damageor incomplete DNA replication. The 9-1-1 complex is recruited byRAD17 to affected sites where it may attract specialized DNApolymerases and other DNA repair effectors. Alternatively splicedtranscript variants of this gene have been described. [provided byRefSeq].
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)Takeishi, Y., et al. Genes Cells 15(7):761-771(2010)Bai, H., et al. DNA Repair (Amst.) 9(5):478-487(2010)Park, M.J., et al. DNA Repair (Amst.) 8(10):1190-1200(2009)Xu, M., et al. J. Biol. Chem. 284(31):20457-20461(2009)
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