Mouse Wnk3 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q80XP9 |
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Clone Names | 110328157 |
Gene ID | 279561 |
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Other Names | Serine/threonine-protein kinase WNK3, Protein kinase lysine-deficient 3, Protein kinase with no lysine 3, Wnk3 |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | Wnk3 {ECO:0000312|MGI:MGI:2652875} |
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Function | Serine/threonine-protein kinase component of the WNK3- SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis and regulatory volume increase in response to hyperosmotic stress (By similarity). WNK3 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid- liquid phase separation (By similarity). The membraneless compartment concentrates WNK3 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK3-dependent phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK (By similarity). Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A4/KCC1, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed:19470686). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A4/KCC1, SLC12A5/KCC2 and SLC12A6/KCC3 inhibits its activity, blocking ion efflux (By similarity). Phosphorylates WNK4, possibly regulating the activity of SLC12A3/NCC (By similarity). May also phosphorylate NEDD4L (By similarity). Also acts as a scaffold protein independently of its protein kinase activity: negatively regulates cell membrane localization of various transporters and channels, such as KCNJ1 and SLC26A9 (By similarity). Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (By similarity). |
Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q9BYP7}. Note=Mediates formation and localizes to cytoplasmic membraneless compartment in response to hyperosmotic stress {ECO:0000250|UniProtKB:Q9H4A3} |
Tissue Location | Expressed in pancreatic duct (PubMed:21317537). |
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Provided below are standard protocols that you may find useful for product applications.
Background
WNK3 (WNK lysine deficient protein kinase 3) belongs to the serine/threonine protein kinase family. It lacks the almost invariant catalytic lysine in subdomain II, which is important for binding ATP in the catalytic site. A conserved lysine in subdomain I is thought to provide this function. WNK3 activates Na-(K)-Cl cotransporters by increasing their phosphorylation and appears to be a crucial component of the kinase/phosphatase signaling pathway that coordinately regulates the Cl? influx and efflux branches of the SLC12A cotransporter family.
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