|Other Names||DNA topoisomerase 2-binding protein 1, DNA topoisomerase II-beta-binding protein 1, TopBP1, DNA topoisomerase II-binding protein 1, TOPBP1, KIAA0259|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double- stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1- responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR (PubMed:16530042).|
|Cellular Location||Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Note=Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation Localizes to sites of DNA damage in a H2AX- independent manner|
|Tissue Location||Highly expressed in heart, brain, placenta, lung and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
TOPBP1 is a binding protein which interacts withthe C-terminal region of topoisomerase II beta. This interactionsuggests a supportive role for this protein in the catalyticreactions of topoisomerase II beta through transient breakages ofDNA strands.
Huo, Y.G., et al. Biochem. Biophys. Res. Commun. 401(3):401-405(2010)Blackford, A.N., et al. Proc. Natl. Acad. Sci. U.S.A. 107(27):12251-12256(2010)Takeishi, Y., et al. Genes Cells 15(7):761-771(2010)Gong, Z., et al. Mol. Cell 37(3):438-446(2010)Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(1):251-257(2010)
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