|Other Names||Diphthamide biosynthesis protein 1, DPH1 homolog, HsDph1, Diphthamide biosynthesis protein 2 homolog-like 1, DPH-like 1, DPH2-like 1, Diphthamide biosynthesis protein 2-like, Ovarian cancer-associated gene 1 protein, DPH1, DPH2L, DPH2L1, OVCA1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||DPH2L, DPH2L1, OVCA1|
|Function||Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers (By similarity). Plays a role in embryonic growth, organogenesis and postnatal survival (By similarity).|
|Cellular Location||Nucleus. Cytoplasm. Note=Punctate, primarily perinuclear localization|
|Tissue Location||Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, mammary gland, colon, small intestine, testis and ovary. Reduced expression in primary breast and ovarian tumors|
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Provided below are standard protocols that you may find useful for product applications.
Diphthamide is a unique posttranslationally modifiedhistidine found only in translation elongation factor-2 (EEF2; MIM130610). This modification is conserved from archaebacteria tohumans and serves as the target for ADP-ribosylation andinactivation of EEF2 by diphtheria toxin (DT) and Pseudomonasexotoxin A. DPH1 is 1 of several enzymes involved in synthesis ofdiphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[suppliedby OMIM].
Liu, S., et al. Mol. Cell. Biol. 24(21):9487-9497(2004)Cardoso, C., et al. Am. J. Hum. Genet. 72(4):918-930(2003)Chen, C.M., et al. Biochem. Biophys. Res. Commun. 286(5):1019-1026(2001)Salicioni, A.M., et al. Genomics 69(1):54-62(2000)Bruening, W., et al. Cancer Res. 59(19):4973-4983(1999)
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