|Other Names||Dematin, Dematin actin-binding protein, Erythrocyte membrane protein band 49, DMTN, DMT, EPB49|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Membrane-cytoskeleton-associated protein with F-actin- binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphorylation by the cAMP-dependent protein kinase A (PKA). Binds to the erythrocyte membrane glucose transporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches the spectrin-actin network to the erythrocytic plasma membrane. Plays a role in maintaining the functional integrity of PKA-activated erythrocyte shape and the membrane mechanical properties. Plays also a role as a modulator of actin dynamics in fibroblasts; acts as a negative regulator of the RhoA activation pathway. In platelets, functions as a regulator of internal calcium mobilization across the dense tubular system that affects platelet granule secretion pathways and aggregation. Also required for the formation of a diverse set of cell protrusions, such as filopodia and lamellipodia, necessary for platelet cell spreading, motility and migration. Acts as a tumor suppressor and inhibits malignant cell transformation.|
|Cellular Location||Cytoplasm. Cytoplasm, cytosol. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Cell membrane. Membrane. Endomembrane system. Cell projection. Note=Localized at the spectrin-actin junction of erythrocyte plasma membrane. Localized to intracellular membranes and the cytoskeletal network. Localized at intracellular membrane-bounded organelle compartment in platelets that likely represent the dense tubular network membrane. Detected at the cell membrane and at the parasitophorous vacuole in malaria-infected erythrocytes at late stages of plasmodium berghei or falciparum development|
|Tissue Location||Expressed in platelets (at protein level). Expressed in heart, brain, lung, skeletal muscle, and kidney|
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Dematin, or EPB49, is an actin-bundling protein originallyidentified in the erythroid membrane skeleton. Its actin-bundlingactivity is abolished upon phosphorylation by cAMP-dependentprotein kinase and is restored after dephosphorylation (Rana etal., 1993 [PubMed 8341682]).
Vugmeyster, L., et al. J. Biomol. NMR 47(2):155-162(2010)Chen, L., et al. Protein Sci. 18(3):629-636(2009)Vugmeyster, L., et al. J. Biomol. NMR 43(1):39-50(2009)Vermeulen, W., et al. J. Mol. Biol. 359(5):1277-1292(2006)Frank, B.S., et al. J. Biol. Chem. 279(9):7909-7916(2004)
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