|Other Names||F-box only protein 5, Early mitotic inhibitor 1, FBXO5, EMI1, FBX5|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding.|
|Cellular Location||Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Note=In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle|
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This gene encodes a member of the F-box protein familywhich is characterized by an approximately 40 amino acid motif, theF-box. The F-box proteins constitute one of the four subunits ofthe ubiquitin protein ligase complex called SCFs(SKP1-cullin-F-box), which function in phosphorylation-dependentubiquitination. The F-box proteins are divided into 3 classes: Fbwscontaining WD-40 domains, Fbls containing leucine-rich repeats, andFbxs containing either different protein-protein interactionmodules or no recognizable motifs. The protein encoded by this genebelongs to the Fbxs class. This protein is similar to xenopus earlymitotic inhibitor-1 (Emi1), which is a mitotic regulator thatinteracts with Cdc20 and inhibits the anaphase promoting complex.Alternatively spliced transcript variants encoding differentisoforms have been identified.
Lei, S.F., et al. J. Bone Miner. Res. (2010) In press :Ma, H.T., et al. Mol. Cell. Biol. 29(24):6500-6514(2009)Lee, J., et al. Mol. Biol. Cell 20(7):1891-1902(2009)Gutgemann, I., et al. Mod. Pathol. 21(4):445-454(2008)Iwai, H., et al. Cell 130(4):611-623(2007)
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