|Other Names||DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1, TOP3B, TOP3B1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.|
|Tissue Location||Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a DNA topoisomerase, an enzyme thatcontrols and alters the topologic states of DNA duringtranscription. This enzyme catalyzes the transient breaking andrejoining of a single strand of DNA which allows the strands topass through one another, thus relaxing the supercoils and alteringthe topology of DNA. The enzyme interacts with DNA helicase SGS1and plays a role in DNA recombination, cellular aging andmaintenance of genome stability. Alternative splicing of theC-terminus of this gene results in three transcript variants whichhave distinct tissue specificity; however, not all variants havebeen fully described.
Oliveira-Costa, J.P., et al. Hum. Pathol. 41(11):1624-1630(2010)Lesch, K.P., et al. J Neural Transm 115(11):1573-1585(2008)Cho, Y.H., et al. Biochim. Biophys. Acta 1679(3):272-278(2004)Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :
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