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TOP3B Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | O95985 |
|---|---|
| Clone Names | 100528220 |
| Gene ID | 8940 |
|---|---|
| Other Names | DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1, TOP3B, TOP3B1 |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | TOP3B |
|---|---|
| Synonyms | TOP3B1 |
| Function | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)- enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity. |
| Tissue Location | Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a DNA topoisomerase, an enzyme thatcontrols and alters the topologic states of DNA duringtranscription. This enzyme catalyzes the transient breaking andrejoining of a single strand of DNA which allows the strands topass through one another, thus relaxing the supercoils and alteringthe topology of DNA. The enzyme interacts with DNA helicase SGS1and plays a role in DNA recombination, cellular aging andmaintenance of genome stability. Alternative splicing of theC-terminus of this gene results in three transcript variants whichhave distinct tissue specificity; however, not all variants havebeen fully described.
References
Oliveira-Costa, J.P., et al. Hum. Pathol. 41(11):1624-1630(2010)Lesch, K.P., et al. J Neural Transm 115(11):1573-1585(2008)Cho, Y.H., et al. Biochim. Biophys. Acta 1679(3):272-278(2004)Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :
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