|Other Names||CST complex subunit STN1, Oligonucleotide/oligosaccharide-binding fold-containing protein 1, Suppressor of cdc thirteen homolog, OBFC1, STN1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha (PubMed:22964711, PubMed:22763445). The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re- initiation of DNA replication at repaired forks and/or dormant origins (PubMed:25483097). Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres (PubMed:22863775, PubMed:22964711). Promotes general replication start following replication-fork stalling implicating new origin firing (PubMed:22863775). May be in involved in C-strand fill-in during late S/G2 phase independent of ot its role in telomere duplex replication (PubMed:23142664).|
|Cellular Location||Nucleus. Chromosome, telomere|
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Provided below are standard protocols that you may find useful for product applications.
OBFC1 and C17ORF68 (MIM 613129) are subunits of an alphaaccessory factor (AAF) that stimulates the activity of DNApolymerase-alpha-primase (see MIM 176636), the enzyme thatinitiates DNA replication (Casteel et al., 2009 [PubMed 19119139]).OBFC1 also appears to function in a telomere-associated complexwith C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009[PubMed 19854130]).
Levy, D., et al. Proc. Natl. Acad. Sci. U.S.A. 107(20):9293-9298(2010)Miyake, Y., et al. Mol. Cell 36(2):193-206(2009)Wan, M., et al. J. Biol. Chem. 284(39):26725-26731(2009)Casteel, D.E., et al. J. Biol. Chem. 284(9):5807-5818(2009)Lamesch, P., et al. Genomics 89(3):307-315(2007)
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