|Other Names||E3 ubiquitin/ISG15 ligase TRIM25, 632n3, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147, TRIM25, EFP, RNF147, ZNF147|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||EFP, RNF147, ZNF147|
|Function||Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).|
|Cellular Location||Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies|
|Tissue Location||Expressed in breast tumors (at protein level). Ubiquitous.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of thetripartite motif (TRIM) family. The TRIM motif includes threezinc-binding domains, a RING, a B-box type 1 and a B-box type 2,and a coiled-coil region. The protein localizes to the cytoplasm.The presence of potential DNA-binding anddimerization-transactivation domains suggests that this protein mayact as a transcription factor, similar to several other members ofthe TRIM family. Expression of the gene is upregulated in responseto estrogen, and it is thought to mediate estrogen actions inbreast cancer as a primary response gene.
Dai, H., et al. Oncol. Rep. 23(3):795-799(2010)Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :Gack, M.U., et al. Cell Host Microbe 5(5):439-449(2009)Ludwig, S., et al. Cell Host Microbe 5(5):420-421(2009)Dai, H., et al. Oncol. Rep. 21(2):395-401(2009)
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