|Other Names||Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.|
|Tissue Location||Expressed in the retina.|
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The protein encoded by this gene is a negative regulatorof the cell cycle and was the first tumor suppressor gene found.The encoded protein also stabilizes constitutive heterochromatin tomaintain the overall chromatin structure. The active,hypophosphorylated form of the protein binds transcription factorE2F1. Defects in this gene are a cause of childhood cancerretinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Liao, C.C., et al. J. Biol. Chem. 285(43):33134-33143(2010)Kim, T.R., et al. Biochem. Biophys. Res. Commun. 400(1):100-105(2010)Hirschi, A., et al. Nat. Struct. Mol. Biol. 17(9):1051-1057(2010)Tooley, C.E., et al. Nature 466(7310):1125-1128(2010)Dimaras, H., et al. Transl Res 156(2):91-97(2010)
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