|Other Names||5'-AMP-activated protein kinase subunit gamma-3, AMPK gamma3, AMPK subunit gamma-3, PRKAG3, AMPKG3|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.|
|Tissue Location||Skeletal muscle, with weak expression in heart and pancreas|
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The protein encoded by this gene is a regulatory subunitof the AMP-activated protein kinase (AMPK). AMPK is a heterotrimerconsisting of an alpha catalytic subunit, and non-catalytic betaand gamma subunits. AMPK is an important energy-sensing enzyme thatmonitors cellular energy status. In response to cellular metabolicstresses, AMPK is activated, and thus phosphorylates andinactivates acetyl-CoA carboxylase (ACC) and beta-hydroxybeta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved inregulating de novo biosynthesis of fatty acid and cholesterol. Thissubunit is one of the gamma regulatory subunits of AMPK. It isdominantly expressed in skeletal muscle. Studies of the pigcounterpart suggest that this subunit may play a key role in theregulation of energy metabolism in skeletal muscle. [provided byRefSeq].
Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010)Jassim, G., et al. Pharmacopsychiatry (2010) In press :Crawford, S.A., et al. Diabetologia 53(9):1986-1997(2010)Ramanathan, L., et al. Protein Expr. Purif. 70(1):13-22(2010)McGeachie, M., et al. Circulation 120(24):2448-2454(2009)
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