|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1701b was selected from the C-term region of human HRH4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H4 belongs to the family 1 of G protein-coupled receptors and has the highest homology to histamine receptor H3 among known G protein-coupled receptors.
Lippert, U., et al., J. Invest. Dermatol. 123(1):116-123 (2004).Coge, F., et al., Biochem. Biophys. Res. Commun. 284(2):301-309 (2001).Morse, K.L., et al., J. Pharmacol. Exp. Ther. 296(3):1058-1066 (2001).Zhu, Y., et al., Mol. Pharmacol. 59(3):434-441 (2001).Liu, C., et al., Mol. Pharmacol. 59(3):420-426 (2001).
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