|Other Names||RNA polymerase II-associated factor 1 homolog, hPAF1, Pancreatic differentiation protein 2, PAF1, PD2|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro.|
|Cellular Location||Nucleus. Note=Punctuate distribution throughout the nucleus except in nucleoli and the perinuclear chromatin|
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Provided below are standard protocols that you may find useful for product applications.
PAF1, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507),and CTR9 (MIM 609366) form the PAF protein complex that associateswith the RNA polymerase II subunit POLR2A (MIM 180660) and with ahistone methyltransferase complex (Rozenblatt-Rosen et al., 2005[PubMed 15632063]). The PAF complex also has a role in histonemonoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[suppliedby OMIM].
Muntean, A.G., et al. Cancer Cell 17(6):609-621(2010)Kim, J., et al. Cell 140(4):491-503(2010)Moniaux, N., et al. PLoS ONE 4 (9), E7077 (2009) :Olsen, J.V., et al. Cell 127(3):635-648(2006)Moniaux, N., et al. Oncogene 25(23):3247-3257(2006)
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