|Other Names||Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10, Ubiquitin thioesterase 10, Ubiquitin-specific-processing protease 10, USP10, KIAA0190|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34- containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989).|
|Cellular Location||Cytoplasm. Nucleus. Early endosome. Note=Cytoplasmic in normal conditions (PubMed:20096447). After DNA damage, translocates to the nucleus following phosphorylation by ATM (PubMed:20096447)|
|Tissue Location||Widely expressed.|
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Ubiquitin is a highly conserved protein that is covalentlylinked to other proteins to regulate their function anddegradation. This gene encodes a member of the ubiquitin-specificprotease family of cysteine proteases. The enzyme specificallycleaves ubiquitin from ubiquitin-conjugated protein substrates. Theprotein is found in the nucleus and cytoplasm. It functions as aco-factor of the DNA-bound androgen receptor complex, and isinhibited by a protein in the Ras-GTPase pathway. The human genomecontains several pseudogenes similar to this gene. [provided byRefSeq].
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Yuan, J., et al. Cell 140(3):384-396(2010)Bomberger, J.M., et al. J. Biol. Chem. 284(28):18778-18789(2009)Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008)Olsen, J.V., et al. Cell 127(3):635-648(2006)
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