|Other Names||DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, RPB16, POLR2D|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes the fourth largest subunit of RNApolymerase II, the polymerase responsible for synthesizingmessenger RNA in eukaryotes. In yeast, this polymerase subunit isassociated with the polymerase under suboptimal growth conditionsand may have a stress protective role. A sequence for a ribosomalpseudogene is contained within the 3' untranslated region of thetranscript from this gene.
Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)Meka, H., et al. Nucleic Acids Res. 33(19):6435-6444(2005)Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003)Kaehlcke, K., et al. Mol. Cell 12(1):167-176(2003)Shilatifard, A., et al. Annu. Rev. Biochem. 72, 693-715 (2003) :
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