|Other Names||Kelch-like protein 12, CUL3-interacting protein 1, DKIR homolog, hDKIR, KLHL12, C3IP1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport (PubMed:22358839, PubMed:27565346). The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed:22358839, PubMed:27565346). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3 (PubMed:16547521). The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4 (PubMed:18303015, PubMed:20100572).|
|Cellular Location||Cytoplasmic vesicle, COPII-coated vesicle|
|Tissue Location||Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland|
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Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3.
Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009)Rondou, P., et al. J. Biol. Chem. 283(17):11083-11096(2008)Lim, J., et al. Cell 125(4):801-814(2006)Angers, S., et al. Nat. Cell Biol. 8(4):348-357(2006)Uchida, K., et al. Immunology 116(1):53-63(2005)
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