|Other Names||Vacuolar-sorting protein SNF8, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22, hVps22, SNF8, EAP30|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).|
|Cellular Location||Cytoplasm. Endosome membrane. Nucleus. Late endosome membrane. Note=Recruited to the endosome membrane to participate in vesicle formation|
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Provided below are standard protocols that you may find useful for product applications.
SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) formESCRT-II (endosomal sorting complex required for transport II), acomplex involved in endocytosis of ubiquitinated membrane proteins.SNF8, VPS25, and VPS36 are also associated in a multiproteincomplex with RNA polymerase II elongation factor (ELL; MIM 600284)(Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001[PubMed 11278625]).
Malerod, L., et al. Traffic 8(11):1617-1629(2007)Lamesch, P., et al. Genomics 89(3):307-315(2007)Langelier, C., et al. J. Virol. 80(19):9465-9480(2006)Bowers, K., et al. J. Biol. Chem. 281(8):5094-5105(2006)Slagsvold, T., et al. J. Biol. Chem. 280(20):19600-19606(2005)
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