|Other Names||Centromere protein U, CENP-U, Centromere protein of 50 kDa, CENP-50, Interphase centromere complex protein 24, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, Polo-box-interacting protein 1, CENPU, ICEN24, KLIP1, MLF1IP, PBIP1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||ICEN24, KLIP1, MLF1IP, PBIP1|
|Function||Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.|
|Cellular Location||Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Note=Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates|
|Tissue Location||Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells Expressed at high levels in glioblastoma cell lines|
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The centromere is a specialized chromatin domain, presentthroughout the cell cycle, that acts as a platform on which thetransient assembly of the kinetochore occurs during mitosis. Allactive centromeres are characterized by the presence of long arraysof nucleosomes in which CENPA (MIM 117139) replaces histone H3 (seeMIM 601128). MLF1IP, or CENPU, is an additional factor required forcentromere assembly (Foltz et al., 2006 [PubMed16622419]).
Suzuki, H., et al. Anticancer Res. 27 (3B), 1423-1430 (2007) :Kang, Y.H., et al. Mol. Cell 24(3):409-422(2006)Izuta, H., et al. Genes Cells 11(6):673-684(2006)Okada, M., et al. Nat. Cell Biol. 8(5):446-457(2006)Foltz, D.R., et al. Nat. Cell Biol. 8(5):458-469(2006)
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