KRIT1 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | O00522 |
---|---|
Clone Names | 100311185 |
Gene ID | 889 |
---|---|
Other Names | Krev interaction trapped protein 1, Krev interaction trapped 1, Cerebral cavernous malformations 1 protein, KRIT1, CCM1 |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | KRIT1 |
---|---|
Synonyms | CCM1 |
Function | Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity (By similarity). Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH- dependent and independent manner, and inhibits ERK1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction, and cell junction stabilization. Plays a role in integrin signaling via its interaction with ITGB1BP1; this prevents the interaction between ITGB1 and ITGB1BP1. Microtubule- associated protein that binds to phosphatidylinositol 4,5-bisphosphate (PIP2)-containing membranes in a GTP-bound RAP1-dependent manner. Plays an important role in the maintenance of the intracellular reactive oxygen species (ROS) homeostasis to prevent oxidative cellular damage. Regulates the homeostasis of intracellular ROS through an antioxidant pathway involving FOXO1 and SOD2. Facilitates the down-regulation of cyclin-D1 (CCND1) levels required for cell transition from proliferative growth to quiescence by preventing the accumulation of intracellular ROS through the modulation of FOXO1 and SOD2 levels. May play a role in the regulation of macroautophagy through the down- regulation of the mTOR pathway (PubMed:26417067). |
Cellular Location | Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. Cell junction. Note=KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions. Association with RAP1 relocalizes KRIT1 from microtubules to cell junction membranes. Translocates from the cytoplasm along microtubules to the cell membrane in a ITGB1BP1-dependent manner |
Tissue Location | Low levels in brain. Very weak expression found in heart and muscle. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a protein containing four ankyrinrepeats, a band 4.1/ezrin/radixin/moesin (FERM) domain, andmultiple NPXY sequences. The encoded protein is localized in thenucleus and cytoplasm. It binds to integrin cytoplasmicdomain-associated protein-1 alpha (ICAP1alpha), and plays acritical role in beta1-integrin-mediated cell proliferation. Itassociates with junction proteins and RAS-related protein 1A(Rap1A), which requires the encoded protein for maintaining theintegrity of endothelial junctions. It is also amicrotubule-associated protein and may play a role in microtubuletargeting. Mutations in this gene result in cerebral cavernousmalformations. Multiple alternatively spliced transcript variantshave been found for this gene.
References
Reddy, S., et al. Graefes Arch. Clin. Exp. Ophthalmol. 248(9):1359-1361(2010)Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Stockton, R.A., et al. J. Exp. Med. 207(4):881-896(2010)Petersen, T.A., et al. AJNR Am J Neuroradiol 31(2):377-382(2010)Lee, Y.W., et al. Ann. Clin. Lab. Sci. 40(3):290-294(2010)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.