|Other Names||Rho guanine nucleotide exchange factor 4, APC-stimulated guanine nucleotide exchange factor 1, Asef, Asef1, ARHGEF4, KIAA1112|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.|
|Cellular Location||Isoform 3: Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated with membrane ruffles.|
|Tissue Location||Expressed at high levels in the brain, skeletal muscle and testis and at low levels in the kidney, lung, small intestine, ovary and prostate. Expression is aberrantly enhanced in most colorectal tumors.|
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Provided below are standard protocols that you may find useful for product applications.
Rho GTPases play a fundamental role in numerous cellularprocesses that are initiated by extracellular stimuli that workthrough G protein coupled receptors. The encoded protein may formcomplex with G proteins and stimulate Rho-dependent signals. Thisprotein is similar to rat collybistin protein. Alternative splicingof this gene generates two transcript variants which encodedifferent isoforms. Also there is possibility for the usage ofmultiple polyadenylation sites for this gene.
Lyons, R., et al. Leuk. Res. 34(1):109-115(2010)Kawasaki, Y., et al. J. Biol. Chem. 284(33):22436-22443(2009)Itoh, R.E., et al. J. Cell. Sci. 121 (PT 16), 2635-2642 (2008) :Kurzik-Dumke, U., et al. Cell. Signal. 19(9):1973-1985(2007)Kuraguchi, M., et al. PLoS Genet. 2 (9), E146 (2006) :
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