|Other Names||Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.|
|Cellular Location||Apical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Membrane raft. Membrane; Single-pass type I membrane protein. Note=In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans- Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells (By similarity). Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1 Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity).|
|Tissue Location||Glomerular epithelium cell (podocyte).|
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This gene encodes a member of the sialomucin proteinfamily. The encoded protein was originally identified as animportant component of glomerular podocytes. Podocytes are highlydifferentiated epithelial cells with interdigitating foot processescovering the outer aspect of the glomerular basement membrane.Other biological activities of the encoded protein include: bindingin a membrane protein complex with Na+/H+ exchanger regulatoryfactor to intracellular cytoskeletal elements, playing a role inhematopoetic cell differentiation, and being expressed in vascularendothelium cells and binding to L-selectin.
Schopperle, W.M., et al. Biochem. Biophys. Res. Commun. 398(3):372-376(2010)Hsu, Y.H., et al. Am. J. Pathol. 176(6):3050-3061(2010)Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :Drossopoulou, G.I., et al. Am. J. Physiol. Renal Physiol. 297 (3), F594-F603 (2009) :Thomas, S.N., et al. Am. J. Physiol., Cell Physiol. 296 (3), C505-C513 (2009) :
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