|Other Names||Piwi-like protein 2, Cancer/testis antigen 80, CT80, PIWIL2, HILI|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (By similarity).|
|Cellular Location||Cytoplasm. Note=Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis (By similarity)|
|Tissue Location||Expressed in adult testis and in most tumors.|
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Provided below are standard protocols that you may find useful for product applications.
PIWIL2 belongs to the Argonaute family of proteins, whichfunction in development and maintenance of germline stem cells(Sasaki et al., 2003 [PubMed 12906857]).
Lee, J.H., et al. Cancer Res. 70(11):4569-4579(2010)Liu, J.J., et al. Int J Clin Exp Pathol 3(4):328-337(2010)Nikpour, P., et al. Cancer Epidemiol 33 (3-4), 271-275 (2009) :Lee, J.H., et al. Hum. Mol. Genet. 15(2):201-211(2006)Kuramochi-Miyagawa, S., et al. Development 131(4):839-849(2004)
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