|Other Names||Nucleoporin p54, 54 kDa nucleoporin, NUP54|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1924a was selected from the Center region of human NUP54. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane.|
|Cellular Location||Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note=Biased towards cytoplasmic side. Central region of the nuclear pore complex, within the transporter (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. NUP54 is a member of the phe-gly (FG) repeat-containing nucleoporin subset.
Stoffler, D., et al., Curr. Opin. Cell Biol. 11(3):391-401 (1999).Bodoor, K., et al., Biochem. Cell Biol. 77(4):321-329 (1999).Popov, S., et al., J. Biol. Chem. 273(21):13347-13352 (1998).Hu, T., et al., J. Cell Biol. 134(3):589-601 (1996).Finlay, D.R., et al., J. Cell Biol. 114(1):169-183 (1991).
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