|Other Names||Zinc finger protein 622, Zinc finger-like protein 9, ZNF622, ZPR9|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1952b was selected from the C-term region of human ZNF622. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||May behave as an activator of the bound transcription factor, MYBL2, and be involved in embryonic development.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Expressed in lung, kidney, spleen, liver and brain with lowest expression in kidney|
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ZNF622 interacts with murine Melk and with itself when expressed in 293T cells. ZNF622 was phosphorylated by Melk both in vitro and in vivo. When expressed alone, ZNF622 exhibited cytosolic and nuclear localization; however, coexpression of ZNF622 with Melk resulted in nuclear localization of ZNF622. ZNF622 also interacts with the transcription factor BMYB in a yeast 2-hybrid assay and coimmunoprecipitated with BMYB when expressed in 293T cells. ZNF622 enhancs the transcriptional activity of BMYB on a reporter plasmid in a dose-dependent manner, and coexpression of ZNF622 and BMYB in 293T cells caused accumulation of both proteins in the nucleus. Constitutive expression of ZNF622 in retinoic acid-treated neuroblastoma cells or 293T cells induced apoptosis, but coexpression of ZNF622 with BMYB inhibited this apoptosis.
Seong, H.A., et al., J. Biol. Chem. 278(11):9655-9662 (2003). Seong, H.A., et al., Biochem. J. 361 (Pt 3), 597-604 (2002).
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