RPC5 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9NVU0 |
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Clone Names | 50908359 |
Gene ID | 55718 |
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Other Names | DNA-directed RNA polymerase III subunit RPC5, RNA polymerase III subunit C5, DNA-directed RNA polymerase III 80 kDa polypeptide, POLR3E, KIAA1452 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP1956a was selected from the N-term region of human RPC5. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | POLR3E (HGNC:30347) |
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Synonyms | KIAA1452 |
Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:20413673, PubMed:12391170, PubMed:35637192). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Assembles with POLR3D/RPC4 forming a subcomplex that binds the Pol III core. Enables recruitment of Pol III at transcription initiation site and drives transcription initiation from both type 2 and type 3 DNA promoters. Required for efficient transcription termination and reinitiation (PubMed:20413673, PubMed:12391170, PubMed:35637192) (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed:19609254, PubMed:19631370). |
Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
RNA polymerase III synthesizes RNA components of the protein synthesis, pre-mRNA splicing, and tRNA processing apparatuses. The holoenzyme consists of about 15 different subunits. The RPC5 subunit is essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters.
References
Hu, P., et al., Mol. Cell. Biol. 22(22):8044-8055 (2002).Jang, K.L., et al., J. Acquir. Immune Defic. Syndr. 5(11):1142-1147 (1992).
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