|Other Names||Histone-lysine N-methyltransferase mes-4, Maternal-effect sterile protein 4, mes-4|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1965b was selected from the C-term region of human Mes-4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Histone methyltransferase. Dimethylates 'Lys-36' of histone H3, a specific tag for epigenetic transcriptional activation. Plays a central role in early development and is responsible for all H3 'Lys-36' dimethylation until about the 40- cell stage. Indirectly involved in the global inactivation of the X chromosomes in germline cells, possibly by excluding the mes-2- mes-3-mes-6 repressive Polycomb complex from the autosomes (PubMed:12077420, PubMed:16968818). Not related to transcription elongation (PubMed:12077420, PubMed:16968818). Required for small- RNA-induced H3K27 trimethylation (PubMed:26365259).|
|Cellular Location||Nucleus. Chromosome. Note=Specifically associated with the autosomes and with the distal tip of chromosome X. Colocalizes with methylated 'Lys-4' of histone H3|
|Tissue Location||In adults, it is predominantly expressed in the germline, and weakly expressed in intestinal cells|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Mes-4 is a histone methyltransferase. This protein dimethylates 'Lys-36' of histone H3, a specific tag for epigenetic transcriptional activation. Mes-4 plays a central role in early development and is responsible for all H3 'Lys-36' dimethylation until about the 40-cell stage. It appears to be indirectly involved in the global inactivation of the X chromosomes in germ line cells, possibly by excluding the mes-2-mes-3-mes-6 repressive Polycomb complex from the autosomes.
Bender L.B., et al. Development. 2006. 133:3907-3917.Fong Y, et al. Science. 2002. 296(5576):2235-8.Korf I, et al. Development. 1998. 125(13):2469-78. Kelly WG, et al. Development. 1998. 125(13):2451-6.Garvin C, et al. Genetics. 1998. 148(1):167-85.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.