|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1969a was selected from the N-term region of human Rde-4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Double-stranded (ds) RNA is a potent sequence-specific inhibitor of gene function. RNA interference (RNAi) is a cellular defense mechanism that uses double-stranded RNA (dsRNA) as a sequence-specific trigger to guide the degradation of homologous single-stranded RNAs. RNAi is a multistep process involving several proteins and at least one type of RNA intermediate, a population of small 21??5 nt RNAs (called siRNAs) that are initially derived from cleavage of the dsRNA trigger. Genetic studies have implicated several RNA interference-deficient (rde) family members in germline maintenance and development, and several simple loss of function mutants have been identified. Family members rde-1 and rde-4 are required for RNAi but are not essential for organismal viability. While rde-1 and rde-4 are distinct from other RNAi-deficient family members both both for their inability to mobilize transposons and lack of chromosome loss, each appears to have a distinct role in the interference mechanism. Evidence indicates that rde-4 is involved before or during production of siRNAs, whereas rde-1 acts after the siRNAs have been formed.
Tabara H, et al. Cell. 2002. 109(7):861-71. Parrish S,et al. RNA. 2001. 7(10):1397-402. Fagard M, et al. PNAS. 2000. 97(21):11650-4 Ketting RF, et al. Nature. 2000. 404(6775):296-8. Tabara H, et al. Cell. 1999. 99(2):123-32.
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