|Other Accession||Q10728, Q9DBR7, NP_002471.1|
|Other Names||Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit, PPP1R12A, MBS, MYPT1|
|Target/Specificity||The synthetic peptide sequence is selected from aa 27-40 of HUMAN PPP1R12A|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity.|
|Cellular Location||Cytoplasm. Note=Along actomyosin filaments and stress fibers|
|Tissue Location||Expressed in striated muscles, specifically in type 2a fibers (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Shichi, D., et al. J. Biol. Chem. 285(44):33680-33690(2010)
Lontay, B., et al. J. Biol. Chem. 285(38):29357-29366(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Wang, X., et al. PLoS ONE 5 (8), E11934 (2010) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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