|Other Names||5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2014a was selected from the N-term region of human NT5E . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor.|
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Provided below are standard protocols that you may find useful for product applications.
Ecto-5-prime-nucleotidase catalyzes conversion at neutral pH of purine 5-prime mononucleotides to nucleosides. The enzyme consists of a dimer of 2 identical 70 kD subunits tethered by a glycosyl phosphatidyl inositol moeity to the exterior plasma membrane surface. The enzyme, a marker of lymphocyte differentiation is associated in deficiency with a variety of immunodeficiency diseases. The preferred substrate is AMP. The NT5 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations.
Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003).Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002).Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990).Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988).Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002).
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