|Other Names||Teratocarcinoma-derived growth factor 1, Cripto-1 growth factor, CRGF, Epidermal growth factor-like cripto protein CR1, TDGF1, CRIPTO|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2047e was selected from the C-term region of human TDGF1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells (PubMed:27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed:11909953).|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor Secreted. Note=Released from the cell membrane by GPI cleavage.|
|Tissue Location||Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung.|
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The human cDNA for CRIPTO (TDGF1), isolated from a teratocarcinoma cell line, encodes a protein of 188 amino acids. The central section has structural similarities with the human transforming growth factor alpha and epidermal growth factor (EGF). CRIPTO transcripts are detected only in undifferentiated cells and disappear after cell differentiation induced by retinoic acid treatment. The EGF-CFC (Cripto, Frl1, and Cryptic) gene family has an important role in vertebrate development. Cripto is required for germ-layer formation and the correct positioning of the anterior-posterior axis in mic; Cryptic (CFC1) is necessary for assignment of the left-right axis. Prior to gastrulation, Cripto expression is initially symmetric and uniform in the epiblast, and then becomes asymmetric in a proximal-distal gradient. Cripto gene when rendered inactive is fatal in the murine model. Cripto and Cryptic are essential cofactors for Nodal family proteins, which transduce signals for mesoderm development. Cryptic protein is required for appropriate symmetry development in humans. TDGF1, like CFC1, is an EGF-CFC family member and required coreceptor in NODAL signaling, a developmental program implicated in midline, forebrain, and left-right axis development in model organisms. A mutation in the conserved CFC domain of the TDGF1 gene has been identified in a patient with midline anomalies of the forebrain. The mutant protein was inactive in a zebrafish rescue assay.
Parisi, S., et al., J. Cell Biol. 163(2):303-314 (2003).Gray, P.C., et al., Proc. Natl. Acad. Sci. U.S.A. 100(9):5193-5198 (2003).Yan, Y.T., et al., Mol. Cell. Biol. 22(13):4439-4449 (2002).de la Cruz, J.M., et al., Hum. Genet. 110(5):422-428 (2002).Dono, R., et al., Am. J. Hum. Genet. 49(3):555-565 (1991).
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