|Other Names||Growth/differentiation factor 9, GDF-9, GDF9|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2069a was selected from the N-term region of human GDF9 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.|
|Tissue Location||Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level)|
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GDF9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues.Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. GDF9 is expressed in oocytes and is thought to be required for ovarian folliculogenesis.
Liao, W.X., et al., J. Biol. Chem. 278(6):3713-3719 (2003).Vitt, U.A., et al., Biol. Reprod. 67(2):473-480 (2002).Aaltonen, J., et al., J. Clin. Endocrinol. Metab. 84(8):2744-2750 (1999).Dong, J., et al., Nature 383(6600):531-535 (1996).McGrath, S.A., et al., Mol. Endocrinol. 9(1):131-136 (1995).
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