UBE4B Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | O95155 |
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Clone Names | 3061110 |
Gene ID | 10277 |
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Other Names | Ubiquitin conjugation factor E4 B, 632-, UBE4B (HGNC:12500) |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP2111a was selected from the N-term region of human UBE4B . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | UBE4B (HGNC:12500) |
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Function | Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed:17369820). |
Cellular Location | Cytoplasm. Nucleus {ECO:0000250|UniProtKB:Q9ES00} |
Tissue Location | Expressed in differentiated myotubes (at protein level) (PubMed:17369820). Highest expression in ovary, testis, heart and skeletal muscle (PubMed:11802788). Expression is low in colon, thymus and peripheral blood leukocytes (PubMed:11802788). Almost undetectable in lung and spleen (PubMed:11802788) |
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Provided below are standard protocols that you may find useful for product applications.
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE4B represents an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. UBE4B also the strongest candidate among the neuroblastoma tumor suppressors.
References
Ishikawa, K., et al., DNA Res. 5(3):169-176 (1998).Mahoney, J.A., et al., Biochem. J. 361 (Pt 3), 587-595 (2002).
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