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(Mouse) Ezh2 Blocking Peptide (Center)
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q61188 |
|---|
| Gene ID | 14056 |
|---|---|
| Other Names | Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Ezh2, Enx1h |
| Target/Specificity | The synthetic peptide sequence is selected from aa 260-275 of HUMAN Ezh2 |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | Ezh2 {ECO:0000312|MGI:MGI:107940} |
|---|---|
| Synonyms | Enx1h |
| Function | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. EZH2 can also methylate non- histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK- BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription. |
| Cellular Location | Nucleus. Chromosome. Note=Localizes to the inactive X chromosome in trophoblast stem cells. |
| Tissue Location | Present in actively dividing cells (PubMed:19026781). Widely expressed in early embryos (PubMed:19026781) In later embryogenesis, expression restricted to central and peripheral nervous system, liver and thymus (PubMed:19026781). In adult, highest expression in spleen, testis and placenta (PubMed:19026781, PubMed:31451685). Lower levels in intestine, muscle and ovary and very low levels in brain and liver (PubMed:19026781, PubMed:31451685). No expression in heart, thyroid gland, lung and kidney (PubMed:19026781) |
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Background
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
References
Hobert O.,et al.Mech. Dev. 55:171-184(1996).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Laible G.,et al.Mamm. Genome 10:311-314(1999).
Denisenko O.N.,et al.Mol. Cell. Biol. 18:5634-5642(1998).
O'Carroll D.,et al.Mol. Cell. Biol. 21:4330-4336(2001).
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