|Other Names||Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2147a was selected from the C-term region of human USP21 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates (PubMed:10799498). Deubiquitinates BAZ2A/TIP5 leading to its stabilization (PubMed:26100909).|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung|
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Provided below are standard protocols that you may find useful for product applications.
USP21 is a ubiquitin-specific protease, an enzyme that removes ubiquitin from ubiquitinated proteins. The encoded protein belongs to the C19 peptidase family, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. This protein has been reported to be capable of removing NEDD8 from NEDD8 conjugates.
Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003). Gong, L., et al., J. Biol. Chem. 275(19):14212-14216 (2000). Hillier, L.D., et al., Genome Res. 6(9):807-828 (1996). Smith, T.S., et al., Biochim. Biophys. Acta 1490 (1-2), 184-188 (2000).
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