|Other Names||ADP-ribosylation factor GTPase-activating protein 3, ARF GAP 3, ARFGAP3, ARFGAP1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2303a was selected from the N-term region of human ARFGAP3 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.|
|Cellular Location||Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment|
|Tissue Location||Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.|
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ARFGAP3 is an GTPase-activating protein (GAP) probably for ARF1. This protein may be involved in protein secretion and/or vesicle transport. It likely participates in promoting hydrolysis of the ARF1-bound GTP and thus, may be required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Like other ARFGAPs, its activity is sensitive to phospholipids. Location is cytoplasmic, concentrated in the perinuclear region. Highest expression occurs in the endocrine glands (pancreas, pituary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary. ARFGAP3 is expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. It is expressed at lower level in spleen, heart, kidney and liver during development.
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).Zhang, C., et al., Genomics 63(3):400-408 (2000).Dunham, I., et al., Nature 402(6761):489-495 (1999).
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