FDFT1 Antibody (Center) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P37268 |
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Other Accession | FDFT_HUMAN |
Clone Names | 3092622 |
Gene ID | 2222 |
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Other Names | Squalene synthase, SQS, SS, FPP:FPP farnesyltransferase, Farnesyl-diphosphate farnesyltransferase, FDFT1 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP2417b was selected from the Center region of human FDFT1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | FDFT1 |
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Function | Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. |
Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q02769}; Multi-pass membrane protein |
Tissue Location | Widely expressed.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
FDFT1 catalyzes the first step in the cholesterol biosynthetic pathway, the conversion of trans-farnesyldiphosphate to squalene. The loss of promoter activity and response to sterols for FDFT1 is localized to a 69-bp section positioned 131 bp 5-prime to the transcription start site. Sequence analysis of this region shows that it contains a sterol regulatory element-1 (SRE1) previously identified in other sterol regulated genes and 2 putative NF1 binding sites.
References
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).Soltis, D.A., et al., Arch. Biochem. Biophys. 316(2):713-723 (1995).Jiang, G., et al., J. Biol. Chem. 268(17):12818-12824 (1993).Robinson, G.W., et al., Mol. Cell. Biol. 13(5):2706-2717 (1993).Summers, C., et al., Gene 136 (1-2Che), 185-192 (1993).
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