|Other Names||NEDD8 ultimate buster 1, Negative regulator of ubiquitin-like proteins 1, Renal carcinoma antigen NY-REN-18, NUB1, NYREN18|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2510a was selected from the N-term region of human NYREN18 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.|
|Cellular Location||Nucleus. Note=Predominantly nuclear|
|Tissue Location||Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2|
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Provided below are standard protocols that you may find useful for product applications.
NYREN18 is a specific down-regulator of the NEDD8 conjugation system. It recruits NEDD8 and its conjugates to the proteasome for degradation. It also interacts with PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome.
Tanaka, T., et al., J. Biol. Chem. 278(35):32905-32913 (2003).Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).Kito, K., et al., J. Biol. Chem. 276(23):20603-20609 (2001).Kamitani, T., et al., J. Biol. Chem. 276(49):46655-46660 (2001).Scanlan, M.J., et al., Int. J. Cancer 83(4):456-464 (1999).
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