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NMT1 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P30419 |
|---|---|
| Other Accession | NP_066565 |
| Clone Names | 3082810 |
| Gene ID | 4836 |
|---|---|
| Other Names | Glycylpeptide N-tetradecanoyltransferase 1, Myristoyl-CoA:protein N-myristoyltransferase 1, NMT 1, Type I N-myristoyltransferase, Peptide N-myristoyltransferase 1, NMT1, NMT |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP2519a was selected from the N-term region of human NMT1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | NMT1 {ECO:0000303|PubMed:9506952, ECO:0000312|HGNC:HGNC:7857} |
|---|---|
| Function | Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins (PubMed:22865860, PubMed:32686708, PubMed:34999170, PubMed:25255805, PubMed:9353336, PubMed:9506952). Also able to mediate N-terminal lysine myristoylation of proteins: catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3' (PubMed:32103017, PubMed:32111831). Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle (PubMed:32103017). |
| Cellular Location | Cytoplasm. Cytoplasm, cytosol. Membrane; Peripheral membrane protein Note=Copurifies with ribosomes. |
| Tissue Location | Heart, gut, kidney, liver and placenta. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The predicted 498-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases.
References
Lindwasser, O.W., et al., Proc. Natl. Acad. Sci. U.S.A. 99(20):13037-13042 (2002).Shiraishi, T., et al., Biochem. Biophys. Res. Commun. 282(5):1201-1205 (2001).Paillart, J.C., et al., J. Virol. 73(4):2604-2612 (1999).Ono, A., et al., J. Virol. 73(5):4136-4144 (1999).Hermida-Matsumoto, L., et al., J. Virol. 73(3):1902-1908 (1999).
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