|Other Names||Myristoylated alanine-rich C-kinase substrate, MARCKS, Protein kinase C substrate, 80 kDa protein, light chain, 80K-L protein, PKCSL, MARCKS, MACS, PRKCSL|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2521b was selected from the C-term region of human MARCKS . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.|
|Cellular Location||Cytoplasm, cytoskeleton. Membrane; Lipid-anchor|
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Provided below are standard protocols that you may find useful for product applications.
MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
Rauch, M.E., et al., J. Biol. Chem. 277(16):14068-14076 (2002).Aderem, A., Biochem. Soc. Trans. 23(3):587-591 (1995).Rao, P.H., et al., Cytogenet. Cell Genet. 66(4):272-273 (1994).Taniguchi, H., et al., J. Biol. Chem. 268(14):9960-9963 (1993).Blackshear, P.J., J. Biol. Chem. 268(3):1501-1504 (1993).
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