|Other Names||Serine palmitoyltransferase 1, Long chain base biosynthesis protein 1, LCB 1, Serine-palmitoyl-CoA transferase 1, SPT 1, SPT1, SPTLC1, LCB1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2534a was selected from the N-term region of human SPTLC1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1- SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass membrane protein|
|Tissue Location||Widely expressed. Not detected in small intestine.|
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Provided below are standard protocols that you may find useful for product applications.
Serine palmitoyltransferase (SPT) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.
Stachowitz, S., et al., J. Invest. Dermatol. 119(5):1048-1052 (2002).Nicholson, G.A., et al., Am. J. Hum. Genet. 69(3):655-659 (2001).Dawkins, J.L., et al., Nat. Genet. 27(3):309-312 (2001).Bejaoui, K., et al., Nat. Genet. 27(3):261-262 (2001).Perry, D.K., et al., J. Biol. Chem. 275(12):9078-9084 (2000).
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