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SPTLC1 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
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- BACKGROUND
| Primary Accession | O15269 |
|---|---|
| Clone Names | 3092402 |
| Gene ID | 10558 |
|---|---|
| Other Names | Serine palmitoyltransferase 1, Long chain base biosynthesis protein 1, LCB 1, Serine-palmitoyl-CoA transferase 1, SPT 1, SPT1, SPTLC1, LCB1 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP2534a was selected from the N-term region of human SPTLC1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | SPTLC1 |
|---|---|
| Synonyms | LCB1 |
| Function | Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core (PubMed:19416851, PubMed:33558762). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference (PubMed:19416851, PubMed:33558762). The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1- SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA (PubMed:19648650, PubMed:19416851). The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (PubMed:19648650, PubMed:19416851, PubMed:33558761, PubMed:33558762). Required for adipocyte cell viability and metabolic homeostasis (By similarity). |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass membrane protein {ECO:0000250|UniProtKB:O35704} |
| Tissue Location | Widely expressed. Not detected in small intestine. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Serine palmitoyltransferase (SPT) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.
References
Stachowitz, S., et al., J. Invest. Dermatol. 119(5):1048-1052 (2002).Nicholson, G.A., et al., Am. J. Hum. Genet. 69(3):655-659 (2001).Dawkins, J.L., et al., Nat. Genet. 27(3):309-312 (2001).Bejaoui, K., et al., Nat. Genet. 27(3):261-262 (2001).Perry, D.K., et al., J. Biol. Chem. 275(12):9078-9084 (2000).
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