|Other Names||Zinc finger protein 350, KRAB zinc finger protein ZFQR, Zinc finger and BRCA1-interacting protein with a KRAB domain 1, Zinc finger protein ZBRK1, ZNF350, ZBRK1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2547a was selected from the Center region of human ZBRK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional repressor. Binds to a specific sequence, 5'-GGGxxxCAGxxxTTT-3', within GADD45 intron 3.|
|Cellular Location||Nucleus. Nucleus matrix. Note=Associated with the nuclear matrix|
|Tissue Location||Widely expressed.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
ZBRK1 binds to a specific sequence, GGGxxxCAGxxxTTT, within GADD45 intron 3 that supports the assembly of a nuclear complex minimally containing both ZBRK1 and BRCA1. Through this recognition sequence, ZBRK1 represses transcription in a BRCA1-dependent manner. The results revealed a novel corepressor function for BRCA1 and provided a mechanistic basis for the biologic activity of BRCA1 through sequence-specific transcriptional regulation.
Liao, G., et al., J. Virol. 79(1):245-256 (2005).Tan, W., et al., J. Biol. Chem. 279(8):6576-6587 (2004).Yun, J., et al., Mol. Cell. Biol. 23(20):7305-7314 (2003).Rutter, J.L., et al., Hum. Mutat. 22(2):121-128 (2003).Ran, Q., et al., Exp. Cell Res. 263(1):156-162 (2001).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.