|Other Names||Zinc finger protein 350, KRAB zinc finger protein ZFQR, Zinc finger and BRCA1-interacting protein with a KRAB domain 1, Zinc finger protein ZBRK1, ZNF350, ZBRK1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2547b was selected from the C-term region of human ZBRK1 (C-term). A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional repressor. Binds to a specific sequence, 5'-GGGxxxCAGxxxTTT-3', within GADD45 intron 3.|
|Cellular Location||Nucleus. Nucleus matrix. Note=Associated with the nuclear matrix|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
ZBRK1 binds to a specific sequence, GGGxxxCAGxxxTTT, within GADD45 intron 3 that supports the assembly of a nuclear complex minimally containing both ZBRK1 and BRCA1. Through this recognition sequence, ZBRK1 represses transcription in a BRCA1-dependent manner. The results revealed a novel corepressor function for BRCA1 and provided a mechanistic basis for the biologic activity of BRCA1 through sequence-specific transcriptional regulation.
Liao, G., et al., J. Virol. 79(1):245-256 (2005).Tan, W., et al., J. Biol. Chem. 279(8):6576-6587 (2004).Yun, J., et al., Mol. Cell. Biol. 23(20):7305-7314 (2003).Rutter, J.L., et al., Hum. Mutat. 22(2):121-128 (2003).Ran, Q., et al., Exp. Cell Res. 263(1):156-162 (2001).
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