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RCHY1 Antibody (N-term H44) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q96PM5 |
|---|---|
| Clone Names | 80612019 |
| Gene ID | 25898 |
|---|---|
| Other Names | RING finger and CHY zinc finger domain-containing protein 1, 632-, Androgen receptor N-terminal-interacting protein, CH-rich-interacting match with PLAG1, E3 ubiquitin-protein ligase Pirh2, RING finger protein 199, Zinc finger protein 363, p53-induced RING-H2 protein, hPirh2, RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP2796a was selected from the N-term region of human RCHY1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | RCHY1 |
|---|---|
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins, including p53/TP53, TP73, HDAC1 and CDKN1B (PubMed:19483087, PubMed:19043414, PubMed:16914734, PubMed:17721809, PubMed:18006823, PubMed:21994467). Mediates ubiquitination and degradation of p53/TP53; preferentially acts on tetrameric p53/TP53 (PubMed:19483087, PubMed:19043414). Catalyzes monoubiquitinates the translesion DNA polymerase POLH (PubMed:21791603). Involved in the ribosome-associated quality control (RQC) pathway, which mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes: RCHY1 acts downstream of NEMF and recognizes CAT tails associated with stalled nascent chains, leading to their ubiquitination and degradation (PubMed:33909987). |
| Cellular Location | Nucleus. Nucleus speckle. Cytoplasm |
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Provided below are standard protocols that you may find useful for product applications.
Background
RCHY1 protein has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. Gene of RCHY1 is oncogenic because loss of p53 function contributes directly to malignant tumor development.
References
Sheng,Y., Nat. Struct. Mol. Biol. 15 (12), 1334-1342 (2008)Maruyama,S., Mol. Cell. Biochem. 307 (1-2), 73-82 (2008)Duan,S., EMBO J. 26 (13), 3062-3074 (2007)Duan,W., J. Natl. Cancer Inst. 96 (22), 1718-1721 (2004)
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