|Other Names||Protein N-terminal glutamine amidohydrolase, 351-, Protein NH2-terminal glutamine deamidase, N-terminal Gln amidase, Nt(Q)-amidase, WDYHV motif-containing protein 1, WDYHV1, C8orf32, NTAQ1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2809c was selected from the Center region of human WDYHV1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N- terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C- terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine (By similarity).|
|Cellular Location||Cytoplasm, cytosol. Nucleus|
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Lim,J., Cell 125 (4), 801-814 (2006)
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